Long Noncoding-RNA Component of Mitochondrial RNA ProcessingEndoribonuclease Promotes Gastric Cancer Cell Multiplication,Migration, and Invasion

Abstract

Since RMRP was found to target microRNA-766-5p (miR-766-5p) in TNBC cells, it enhanced cell viability and
decreased apoptosis by silencing miR-766-5p. Additionally, RMRP has been proven to be highly involved in
either pathological or physiological processes. Therefore, the study aims to determine whether RMRP promotes
the multiplication, migration, and invasion of gastric cancer cells. The study will overexpress RMRP in GC1401
gastric cancer cells and measure cell growth by MTT assay, migration by wound healing assay, and Boyden chamber
assay. Positive control is another treatment previously shown to increase cancer cell growth and migration, such as
IQGAP3, which, beginning with the early stages of tumor growth, is markedly up-regulated in human stomach cancer.
Furthermore, the negative control is DMSO. The study measures miR206 by qRTPCR. If the solution gets dark through
MTT assay, RMRP promotes gastric cancer cell multiplication. If the migration rate is getting quicker according to the
wound healing assay, and the number of migration cells increases by Boyden chamber assay, RMRP promotes gastric
cancer cell migration. If miR-206 may affect the expression of HIF-1 to control cell growth and ECM buildup, RMRP
promotes gastric cancer cell invasion by acting as a miR-206 sponge for gastric cancer. The RNA component of miRNA
processing endoribonuclease, the novel biomarker for gastric cancer, promotes gastric cancer cell multiplication,
migration, and invasion by acting as a miR-206 loss of function in cell lines for gastric cancer.