The Detection of Inactivated p53 Protein by a Biosensor
DOI:
https://doi.org/10.61173/ex12yq30Keywords:
molecular cloning, green fluorescent protein, P53, artificial enzymeAbstract
P53 is a gene that closely correlates with human tumors. In this paper, we explore how various biology techniques
can be applied to detect the malfunction of p53. Firstly, we insert the GFP gene, as correctly translated p53 protein
will undergo fluorescence. Secondly, we apply artificial enzymes such as an antibody, and the structure of functional
p53 protein differs from those that are incorrectly translated. The enzyme can detect this as its active site matches the
structure of the functional p53 protein. Lastly, we aim to detect not the p53 protein but its side product. Caspase 3 is the
one we will target to find. In this paper, we carefully compare and contrast these three methods, and the choice should
always be considere with different experimental conditions. The detection using GFP should be the best method among
the rest, as it gives a visually accessible result, is also easy, and can produce the result very quickly.