Effects of Nicotine on the Progression and Metastasis of HumanOvarian Cancer cells by Exacerbating EMT

Abstract

Does nicotine, a main constituent of toxicants in Cigarette Smoke, relate to EMT and contribute to the progression of
ovarian cancer? Treat SK-OV3 xenograft mice with increasing nicotine and measure TGF-β in blood by ELISA assay
and tumor cell metastasis by confocal microscopy, looking for cancer cells in the liver, bone, brain, stomach, ovary and
then measure xenograft tumor size by weight. A positive control is benzene treatment, and the negative control is PBS.
The results will reveal the effects of nicotine on ovarian cancer cell proliferation, which will improve our understanding
of the harm environmental toxicants can do to human health. At the same time, the physiological processes that lead to
carcinogenesis will also provide prevention awareness and treatment ideas for ovarian cancer caused by smoking.