Potential Treatment of Non-Small Cell Lung Cancer by UpregulatingZinc Finger Protein 671 Expression

Abstract

Non-small cell lung cancer (NSCLC) is the most common type, with a high mortality rate. It is often diagnosed at an
advanced stage, which causes treatment difficulties due to a lack of symptoms at early stages. Here, we propose a new
approach for treating NSCLC via upregulating zinc finger protein 671 (ZNF671) expression. Firstly, we will reduce the
degree of methylation at its gene promoter site in the NSCLC tumor cells. Secondly, the RT-qPCR technique is used
to compare the expressions of ZNF671 between different methylation statuses; in vitro cell viability, proliferation, and
invasion assays are applied to test the effects of demethylation in tumor cells. Finally, the patient-derived xenografts
(PDX) model is used to observe the effect of regulating methylation status on the expression of ZNF671, affecting tumor
growth in vivo.